The invention discloses agrobacterium-mediated transgenic Sophora japonica and its tissue culture method for rapid multiplication. The method comprises (1) selecting and disinfecting seeds, (2) selecting and cultivating Agrobacterium tumefaciens, (3) cultivating tissue culturing seedlings, (4) infecting with Agrobacterium tumefaciens, (5) selecting resistant strain, (6) identifying transformed plants, (7) breeding, and (8) transplanting seedlings. The invention establishes a regenerating system with high frequency suitable for genetic transformation and a repeatable wood gene transgenic technology system with high transforming efficiency, obtains transgenic plants with improved salt tolerance, and establishes an integrated tissue culture method for rapid multiplication. The method of the invention provides basis for the transformation of agrobacterium-mediated target gene to xylophyta, rosette pullulate differentiation, radication, and transplantation.