The invention provides a detecting Furacilin metabolite ELISA kit, which contains: an ELISA plate covered by the original coated, enzyme marker, Furacilin metabolite specific antibody or Furacilin metabolite derivative antibody, Furacilin metabolite standard solution or Furacilin metabolite derivative standard solution, the substrate color solution, the termination solution, the condensed washingsolution, the condensed complex solution. The invention also provides a method to apply the above ELISA kit detecting Furacilin metabolite, which includes steps: firstly, sample pre-treatment, and then using the kit for testing, and finally analyzing testing results. The invention is to provide the Furacilin metabolite residues in the ELISA kit for detection of animal derived foods, such as chicken, pork, fish, shrimp, milk, honey, egg, and other samples, and the detection method is simple, low cost, high sensitivity, and it can monitor on the scene and suitable for screening large number of samples.