A cellular electrophysiological measurement device (1) includes a thin plate (7) and a frame (6). The thin plate has a first surface (3a) with a depression (8) and a second surface with a through-hole (9). The frame (6) is in contact with an outer periphery (4b) on the second surface of thin plate (7). The thin plate (7) has a laminated structure of at least two layers including a first material layer (3) on first surface (3a) and a second material layer (4) on the second surface. The frame (6) is formed of a third material layer (5). The structure allows the cellular electrophysiological measurement device (1) to be not so vulnerable to breakage of thin plate (7) and other damages, thereby having high production yield.