The invention discloses a measuring device for measuring analyzed matter and the method. The device comprises movable region, intercepting region and measuring region. The movable region comprises special combination analyzed matter molecule with marking material, which can flow as liquid flows. One of special combination molecules not related to analyzed matter is connected on the molecule. A matter like analyzed matter is fixed on the intercepting region and one of special combination molecules not related to analyzed matter is fixed on the measuring region. The invention can display measuring result direction and can accomplish reaction by only one operation. At the same time it can improve measuring sensitivity and accuracy.