The invention relates to a cell freezing device. It comprises temperature control pipe and temperature checking device installed on sample shelf on low- temperature box. It is characterized in that: temperature control pipe is composed of copper pipe, insulating layer and electrical heating wire; copper pipe is installed on sample pipe, the out of copper pipe is covered with insulating layer, the insulating layer is wound with electrical heating wire; temperature control device comprises temperature data gathering device and temperature control device, temperature data gathering device includes temperature control pipe, thermal couple; thermal couple is inserted into temperature control pipe, the thermal electic signal of thermal couple controls electrical heating wire after passing through temperature control pipe. The device can solve problems of biological cell temperature reduction and temperature recover control, and is characterized by high sample temperature evenness, effective temperature recover device, fast and in time response, low production cost, convenient operation, and wide application for programme temperature reduction and recover for various biological materials low- temperature restorage.