The present invention is target cell period gene chip for the research of 120 genes related to cell period regulation. It is found by means of gene chip technology research that H2O2 damages human umbilical vein vascular endothelial cell via up regulating the expression of its 48 cell period genes while ophiopogonin D can down regulate the expression of 39 genes of the said 48 cell period genes and other 15 genes, and H2O2 can down regulate the expression of its 1 cell period gene while ophiopogonin D can down regulate the expression of this gene. Therefore, ophiopogonin D can antagonize the damage of H2O2 on vascular endothelial cell to protect the morphologic and functional integrity of vascular endothelial cell and to prevent and treat cardiac and cerebral vascular diseases.