The present invention discloses a method for inducing mesenchymal stem cell to divide into nerve cell and the special culture medium. The culture medium is DMEM culture medium of cattle fetus blood serum which the volume and the thickness percent ratio is 8 to 12 percent. The method is provided with steps as follows: firstly, a small disturbing RNA which restrains the express of limited silent gene of a nerve cell is composed or an intervention carrier of carrying a small disturbing RNA coding gene with restraining the express of limited silent gene of a nerve cell is composed; secondly, the small disturbing RNA which restrains the express of limited silent gene of a nerve cell or the intervention carrier of carrying a small disturbing RNA coding gene with restraining the express of limited silent gene of a nerve cell is transformed or transfected into mesenchymal stem cell or fat stem cell, then the recombined mesenchymal stem cell or the recombined fat stem cell is positioned in theculture medium induced by nerve cell, and cultivated under the temperature of 36 to 38 DEG C, thereby getting nerve cell. The prevent invention can play an important role in the medical field, and has wide applied prospect.