The invention discloses a high effective agro bacterium genetic conversion method of a heavy metal over enrichment plant Indian leaf mustard, which comprises the following steps: culturing the disinfected mature seed of Indian leaf mustard until a pair of cotyledons expand; inoculating seedling hypocotyls, cotyledon stem or cotyledon in the culture medium and culturing; co-culturing the disseminated agro bacterium in the co-culture medium; transferring to the front culture medium until callus expands; getting the resistant callus by transferring to the screening culture medium; differentiating the resistant overgrow bud; culturing the overgrow bud in the basic culture medium; proceeding with the water and growing-root culture by transferring to HeGELanTe culture liquid; planting by transferring the strong sprout with the root to the nursery base material; getting the transferred gene Indian leaf mustard mature plant. The method can remove the influence of the external condition, whichis fit for four seasons, saves the nursery sock growing field, reduces the cost, acquires a great deal of strong transferred gene seedlings in the short time, and meets the research and the production application of molecular biology.