The invention belongs to the technical field of biology, relating to restructuring DNA molecules of a single replication sequence which is capable of encoding human pancreatic cancer MUC1 and also DNA vaccine inoculation constructed by the DNA molecules and the usage of the vaccine inoculation. The restructuring DNA molecules enable coding genes of the most immunogenic nucleic replication sequences of the MUC1 to be the core. An MCP-I signal peptide gene order is inserted at the amidogen end, simultaneously a promoting eukaryon translating sequence of a KOZAK is inserted in front of an initial code. The restructuring DNA molecules are directionally cloned into a eukaryon expression vector MCS, constructing and restructuring the DNA vaccine inoculation of a mucus core protein peptide of the human pancreatic cancer. After ukaryotic cells transfect the vaccine inoculation of the invention, a VNTR can be expressed in the inner and outer cells. Animal experimental results display that the vaccine inoculation has protective effects against a tumor attack, has treatment effects on a mouse pancreatic cancer of the expression human MUC1, can specifically kill the human pancreatic cancer cells of the expression MUC1, and has the treatment effect on the human pancreatic cancer.