The invention relates to a detecting method for total gastrodin and combined gastrodin. The method comprises the steps as follows: the materials of the detected object which at least comprises the ingredients of combined gastrodin is hydrolyzed under an alkali condition, and then the contents of the dissociative gastrodin ingredients are detected to obtain the contents of master gastrodin in the materials of the detected object, which comprises dissociative gastrodin and combined gastrodin. The contents of the combined gastrodin in the detected object are obtained after deducting the dissociative gastrodin which is obtained through the prior detecting method in the detected object from the master gastrodin, thereby providing a reliable method for objective and accurate detection and quality control on the contents of gastrodin component of raw material or preparation in medicine preparation and/or medicine use, and further being beneficial for ensuring the product quality, increasing the medicine efficiency, and decreasing the adverse influence due to inaccurate contents of active ingredients.