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发明授权
CN101177701B 人乳头状瘤病毒基因分型检测试剂盒及其基因芯片制备方法 有权 许可 转让

人乳头状瘤病毒基因分型检测试剂盒及其基因芯片制备方法
摘要：
本发明公开了一种具有与人乳头状瘤病毒HPV DNA互补的核苷酸序列的探针，和含有该探针的基因分型检测试剂盒及其基因芯片制备方法。该探针选自SEQ ID Nos：1-21和与SEQ ID Nos：1-21互补的序列。该试剂盒包括一个基因芯片，其上带有至少一个上述探针、标记有生物素点的DNA序列、带有编码β-球蛋白部分的DNA序列，各种选自MY09/MY11的引物。该试剂盒的基因芯片通过DNA探针的制备，固定探针，利用氢氧化钠停止反应等步骤制得。本发明的试剂盒能快速、准确地检测出HPV感染及鉴定具体的亚型，更适合中国人，对于子宫颈癌的早期诊断、预防、治疗和手术后的跟踪具有重大的意义。
摘要（英）：

The invention discloses a nucleotide sequence probe which is complementary to human papillomavirus HPV DNA, and a gene typing test kit which is provided with the probe and a preparation method for a gene chip thereof. The probe chooses from a sequence of SEQ ID Nos: 1-21 and a complementary sequence to the SEQ ID Nos: 1-21. The kit comprises the gene chip provided with at least one probe, a DNA sequence which is marked with a biotin point, a DNA sequence with the coding of Beta-globulin and various primers chosen from MY09/MY11. The gene chip of the kit is produced through the steps of: preparing the DNA probe, fixing the probe, stopping reaction under the help of sodium hydroxide, etc. The kit of the invention can fast and exactly detect conditions of HPV infection and identify the concrete subtype, be more suitable for Chinese and have significant meaning for early diagnosis, prevention, treatment of cervical cancer and for tracking treatment after operation.

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