The invention relates to the technical field of medicine, in particular to a novel preparation method of docetaxel injection. The prescription of the docetaxel injection of the invention consists of docetaxel, stabilizing agent and menstruum used for injection. The preparation method includes the following steps: (1) the stabilizing agent of a prescription amount is dissolved in the menstruum of the prescription amount used for injection; (2) the docetaxel of the prescription amount is dissolved in the solution obtained in the step (1); and then filtering, sub-package and sterilization are carried out to finish the preparation. The docetaxel injection of the invention has better stability and convenient use.