The invention provides a refine preparation method of polyglucose. Glucose is solid-phase condensed by catalyst in the conditions of high temperature and acid to obtain polyglucose semiproduct to prepare aqueous solution of the polyglucose semiproduct and then the aqueous solution is added with sorbent, heated and stirred; rough filtration and straining are carried out and hollow fiber ultrafiltration membrane is used for measuring off molecular weight; the filtrate is condensed to obtain polyglucose solid powder by spray drying. The polyglucose product obtained by the method of the inventionis uniform and steady in quality, safe and environment protective in production.