The present invention discloses an extract of acanthopanax effective part, a preparation method thereof and an application thereof. In the acanthopanax extract of the present invention, total glycoside takes 20 to 64wt percent of the gross extract; total flavone takes 20 to 65wt percent of the gross extract; total glycoside total flavone takes 50 to 90wt percent of the gross extract. The preparation method of the acanthopanax effective part of the present invention is simple and convenient and causes little environmental pollution; the extracting and separating carrier can be used repeatedly for a plurality of times; the present invention has low cost and is applied to mass industrialization production.