The present invention has its application in the field of devices and methods for the product physical and chemical mixing and refers particularly to a cartridge for sterile mixing of a two-phase compound. The cartridge consists of a first tubular body (2) defining a first collection chamber (3) for a solid phase, a second tubular body (4) defining a second collection chamber (5) for a liquid phase, means for transferring (6) the liquid phase from the second (5) to the first chamber (3), means for mixing (7) the phases. The means for mixing (7) comprise agitator means (8) acting on the mixture inside the first chamber (3) with the first tubular body (2) in substantially stationary conditions, so as to favour the dispersion of the solid phase inside the liquid phase thus obtaining a compound with uniform physical and mechanical properties.