The invention discloses a new usage of a psoralea fruit extract, in particular to an application of the psoralea fruit extract in preparing antitumor medicines. The extract is prepared by the method containing the following steps: water and/or organic solvent are used as extractant, and the psoralea fruit extract is extracted and obtained from the seeds of psoralea fruit. The new usage of psoralea fruit extract provided by the invention has important significance for preparing antitumor medicines and especially for hepatic cancer resistance medicines.