The invention provides lipophilic slow release fertilizer used for modifying environmental creatures in oil pollution positions and a preparation method for the fertilizer. The composition comprises granular nitrogen fertilizer, calcium dihydrogen phosphate, trace elements, polyvinyl alcohol, vegetable oil and fillers. The granular nitrogen fertilizer is placed in a barrate coating machine; the calcium dihydrogen phosphate, the trace elements and the fillers are mixed uniformly and placed in a feeding tank of the coating machine; the polyvinyl alcohol is prepared as water solution with the concentration of 15 percent, poured into an elevated tank of the coating machine and at the same time rotating speed and the temperature are set; the coating machine and an air compressor are started and the water solution is sprayed on a granular surface of nitrogenous fertilizer according to the set rotating speed and the temperature; meanwhile, the mixture of the calcium dihydrogen phosphate, the trace elements and the fillers are continuously sprinkled and the coating machine and the air compressor are heated to be rotated and the mixture is dried; the vegetable oil is dropped gradually and the two machines continue to rotate and then the coating machine and the air compressors are stopped; the temperature of the mixtures is decreased to room temperature to finish the products. The fertilizer can be attached to the oil and can slowly release the nutrients to maintain the constant growth of bacteria cells, thus remarkably promoting modifying efficiency of the creatures in the oil pollution places.