The invention discloses an application of an acetylcholinesterase piezoelectric biosensor in detecting nerve bundle property. A choline esterase antibody fixed on a quartz crystal reacts to acetylcholinesterase contained in a nerve sample to be measured, the oscillation frequency of the quartz crystal changes in the reaction process, then the content of the acetylcholinesterase is measured and mirrored through a computer monitor system, the nerve bundle to be measured with high content is of a motion tract, and the nerve bundle to be measured with low content is of a sensory tract. The invention starts with the difference of the content of the acetylcholinesterase in the peripheral nerve motion tract and the sensory tract, can fast detect an acetylcholinesterase piezoelectric biosensor system, fast discriminate the peripheral nerve motion tract and the sensory tract, and realize the correct sewing of the motion tract to the motion tract, and the sensory tract to the sensory tract. Theinvention can be widely popularized in the further clinical application.