The invention discloses a bearing device of a glass substrate, comprising a bearing base, buffering row plates and positioning row plates, wherein, a first opposite absorbing part is arranged inside the bearing base; bottoms ends of a plurality of buffering row plates are all provided with a second opposite absorbing part to cause the buffering row plates to be absorbed on the first opposite absorbing part. A plurality of positioning row plates are fixed inside the bearing base; each positioning row plate has a plurality of slots; at least a buffering row plate is arranged between the adjoinedpositioning row plates to position the glass substrate in a the corresponding slot of the adjoined positioning row plate and bear a bottom of the glass substrate through top ends of the buffering rowplates.