The invention discloses a naonparticle preparation of periwinkle alkaloids antitumor drug. The particle diameter is between 10 and 150 nm and the raw materials of the naonparticle preparation comprises by weight percentage: 0.1 to 5 percent of the periwinkle alkaloids antitumor drug, 0.5 to 3 percent of carrier, 0.5 to 3 percent of stabilizer and 89 to 98.5 percent of hydrochloric acid solution with pH value of 2; the sum of the weight percentages of the raw materials is 100 percent. The naonparticle preparation of periwinkle alkaloids antitumor drug can effectively enhance sensitivity to tumor cells and reduce the drug toxicity. The preparation method of the invention has the advantages of quick and easy property, low cost, high maneuverability and comparatively narrow particle size distribution, etc.; organic solutions are not adopted in the preparation process, without problem of organic solution residue.