The invention provides a method for establishing a radiation-induced vitiligo mouse model by applying the different doses of Gamma-ray to carry out whole body irradiation of an IRM-2 mouse. The radiation-induced vitiligo mouse model is mainly used for the research of pathogenesis of vitiligo and the development of drugs for prevention and treatment, which can also be used for the research of tumor immune mechanism and the screening of targets for immunotherapy and the research of radiation-induced immune dysfunction mechanism. The model of the invention is characterized by simple preparation, early lesion emergence time, high incidence, low radiation dose and so on, which further lays the foundation for the experimental research of the pathogenesis of vitiligo and the screening of new drugs.