利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN101288774A 猪附红细胞体的小鼠感染模型的构建方法 无效 - 撤回

猪附红细胞体的小鼠感染模型的构建方法
摘要：
一种生物技术领域的猪附红细胞体的SPF小鼠感染模型的构建方法。包括步骤：(1)猪附红细胞体的分离纯化；(2)SPF小鼠饲养于熏蒸消毒的隔离室中；(3)接种；(4)动物模型检测评估。本发明成功构建了猪附红细胞体的SPF小鼠感染模型，并通过鲜血压片检测，PCR检测和透射电镜检测三种方法验证。本模型可用于附红细胞体病的病原学，致病力，病理学和免疫学等方面的深入研究。
摘要（英）：

The invention provides a construction method of the SPF mouse infection model of an epperythrozoon in the biological technology field, which includes the steps of (1) the separation and purification of the epperythrozoon, (2) the feeding of the SPF mouse in the isolation chamber for the fumigation and the disinfection, (3) the vaccination, (4) the detection evaluation of the animal model. The invention successively constructs the SPF mouse infection model of the epperythrozoon and is verified by three methods, including fresh blood squash detection, PCR detection and transmission electron microscope detection. The model can be applied to the deep researches in the aspects of the etiology, the pathogenic force, the pathology and the immunology of eperythrozoonosis, etc.

0/0