The invention relates to the anti-counterfeiting printing technical field, in particular to a multi-encryption and anti-counterfeiting printing method for a two-dimensional code which is applicable to the two-dimensional code and has encryption requirements on the information of the two-dimensional code, and the multi-encryption and anti-counterfeiting printing method is carried out according to the following steps: (1) original data of the two-dimensional code to be encrypted is input to an encryption system; (2) the encryption system takes out one or a plurality of bits from the original data of the two-dimensional code to carry out a first encryption algorithm calculation, thus obtaining a group of random check codes; (3) the group of random check codes are arranged in the original data of the two-dimensional code, thus generating a group of data containing the random check codes; (4) the obtained data containing the random check codes is carried out a second encryption algorithm calculation, thus generating the dual-encryption data; (5) the obtained dual-encryption data is utilized for generating the dual-encryption two-dimensional code; (6) the dual-encryption two-dimensional code is transmitted to a digital printing system for printing. The method can effectively prevent others from utilizing the existing two-dimensional code reading device to decode the encrypted content, thus obtaining the information in the two-dimensional code and having good confidentiality.