The present invention relates to an antibiotic biological pesticide, a method for preparation and application thereof, characterized in that the active component of the biological pesticide is agricultural antibiotic HA97502 with the molecular formula of C25H37O4N and the chemical structural formula as follows. In preparation, the microorganism zymolysis technique is adopted, wherein the production bacterial is streptomyces aburayiensis HA97502. As an agricultural insecticide, the biological pesticide exhibits specific effects to important destructive insects in agricultural production, such as red spiders, aphids, cabbage worms etc.