The invention discloses a construction method for a plasmid used to express a xylulokinase gene, which relates to the construction method for a plasmid vector. The construction method solves the problems that: the prior saccharomyces cerevisiae altered by metabolic engineering has low xylose utilization rate, and a fermentation byproduct xylitol has high output. The construction method is as follows: a KanR gene, an XKS1 gene, an ADH1 terminator fragment, and a 2.2 kb rDNA fragment in the saccharomyces cerevisiae are cloned; then gene fragments are reclaimed and purified, and then are connected with a pGEM Easy vector; then a plasmid pR, a plasmid pRK and a plasmid pRKT are orderly constructed; finally the 2.2 kb rDNA fragment is added into the plasmid pRKT. The vector constructed by the method can perform high copy integration of the XKS1 gene to a chromosome genome of the saccharomyces cerevisiae, realize the stable overexpression of the XKS1, dredge metabolic flux that the saccharomyces cerevisiae utilizes xylose to produce alcohol, improve the utilization rate of the xylose, and reduce the output of the byproduct xylitol.