The invention relates to an auxiliary fixing device of a laboratory electric pipette gun in the cell culture field, which comprises a gun frame body, a gun frame base, a vertical pipette box and a leakage basin. A gun handle groove and a high elastic baffle are arranged at the top part of the gun frame body, the high elastic baffle is arranged at the rear part of the gun handle groove, the hollow space of the gun handle groove is matched with an universal pipette gun, and a hollow power line groove space is arranged at the bottom end. A notch which is matched with the vertical pipette box is arranged on the gun frame base, and the gun frame base is spliced with the vertical pipette box through vertically inserting; a circular hollow inlaid cup shaped leakage basin is also arranged. The auxiliary fixing device is fixedly laid when the pipette gun is not used, and can prevent being knocked down caused by horizontally laying; after imbibing, the auxiliary fixing device can still be fixedly and momentarily laid according to the requirements so as to avoid irreversible imbibition blowing caused by misoperation; when the opening of the pipetting objective container is undersize and the pipette is long, tapping is slowly performed after the pipette gun and the pipette are fixed; when the leakage due to poor air tightness is casually found after imbibing, the auxiliary fixing device plays the roles of casual buffer and carrying under the fixing condition.