The invention relates to a printing screen which comprises a screen frame, a silk screen and a metal sheet with a screen printing pattern; the silk screen is arranged at the screen frame in a tightening way; the metal sheet is fixed on the surface of the silk screen; the screen printing pattern of the metal sheet is ablated by the laser; the precision of the screen printing pattern is +0.005 to +0.02mm or -0.02 to -0.005mm. The invention also relates to a manufacturing method of the printing screen. The printing screen with high precision is beneficial to a circuit board with high density to carry out precise screen printing, can effectively prevent the scrapping of the circuit board caused by the screen printing and improve the yield of manufacturing the circuit board.