The invention discloses a nucleic acid concentrator and an application thereof. The nucleic acid concentrator is obtained through connecting specific probes on the inner wall of a vessel; from 3' end to 5' end, the specific probes orderly comprise a bonding zone consisting of 25 to 150 ribonucleotides which are complemented with conservative zone of a target DNA or RNA to be tested, as well as a connecting arm consisting of 6 to 30 deoxy ribonucleotides; and the ribonucleotides at 5' end of the connecting arm is modified to be connected with the inner wall of the vessel. The nucleic acid concentrator can be used to concentrate ribonucleic acid or deoxy ribonucleic acid, thereby being applied to polymerase chain reaction (PCR) and nucleic acid testing. While carrying out nucleic acid testing, the nucleic acid concentrator has the advantages of high selectivity, high specificity, one-tube-type fast testing, low cost, strong operability and wide application range, and is especially suitable for fast analysis and testing of the gene in the organization with extremely low nucleic acid content.