The invention discloses a mixed insecticide of paecilomyces javanicus and acetamiprid, which consists of a main agent and an adjuvant; according to a weight percent, the main agent is 22 - 61 percent, and the adjuvant is 39 - 78 percent; the main agent is the mixture of the paecilomyces javanicus and the acetamiprid, and the weight ratio of the paecilomyces javanicus and the acetamiprid is 100:1.5 - 10; and the adjuvant is a stabilizer, a promoter and a filler. The mixed insecticide of the invention can be processed into a wettable powder, a suspending agent or an oil agent; and by long-term infection biology researches and indoor creature tests, the mixed insecticide has strong infection insecticidal effect on a plurality of pests, in particular to the pests with piercing-sucking mouthparts, and has good preventing effect on such pests as diamondback moth, aphid, thrips, etc.