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发明授权
CN101333526B 人巨细胞病毒UL54基因的siRNA序列及应用 失效 - 权利终止

人巨细胞病毒UL54基因的siRNA序列及应用
摘要：
本发明提供一种人巨细胞病毒UL54基因的siRNA的cDNA序列，其核苷酸序列是：5’-CTCGATGAAGTCAAGAAGT-3’。本发明根据siRNA设计原则，选择的siRNA其cDNA序列的GC含量为42.1％，对应UL54mRNA中3058-3076核苷酸位点，构建针对HCMV UL54(DNA多聚酶)基因的siRNA真核表达载体，转化大肠杆菌后提取质粒，分别转染AD293细胞和MRC-5细胞，最终筛选到1个能有效抑制UL54基因表达的siRNA序列，可在制备治疗人巨细胞病毒感染的药物中的应用。
摘要（英）：

The invention provides a cDNA sequence of siRNA aiming at a cytomegalovirus UL54 gene of human, and the nucleotide sequence is: 5`-CTCGATGAAGTCAAGAAGT-3`. According to the design principle of the siRNA, the cDNA sequence of the selected siRNA has a GC content of 42.1 percent, and the selected siRNA is corresponding to 3058-3076 nucleotide sites in UL54 mRNA, siRNA eukaryotic expression vectors aiming at the HCMV UL54 (DNA polymerase) gene are constructed, and plasmids are extracted after transforming Escherichia coli for respectively transfecting AD293 cells and MRC-5 cells, thereby finally screening out one siRNA sequence that can effectively inhibit the UL54 gene expression, and the sequence can be applied to the preparation of drugs for curing cytomegalovirus infection of human.

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