The invention provides an expression type pre-T-vector for quickly cloning and expressing the product PCR of the target gene, and a preparation and an application thereof. The advantages of the expression type pre-T-vector mainly lie as follows: the target gene can be cloned and expressed by one step method, thereby omitting complicated recombination processes; the product PCR is not needed to bepurified and enzyme cut in construction process, and linearized T-vector can be obtained by the method that the pre-T-vector is single enzyme cut by XcmI with high enzyme cutting efficiency without using other restrictively restriction enzymes; the target gene is not needed to be induced by expensive IPTG, which can ferment engineering bacteria in large scale with lower cost; and the sequencing can be identified after the target gene is expressed, and then transformants whose expression results are up to expectation are sent to the DNA sequencing company to be sequenced, so the sequencing success rate can be improved, and the expenses can be saved.