The invention provides a measurement method of the content of morinda officinalis oligosaccharides, which comprises the steps as follows: the high-performance liquid chromatography is adopted for carrying out the measurement; water solution containing acetonitrile is taken as mobile phase, the contents of 3 to 9 polymers of inulin-typed oligosaccharides are respectively calculated by peak areas according to the external standard method, and the contents of the 3 to 9 polymers of the inulin-typed oligosaccharides are summed to obtain the content of the morinda officinalis oligosaccharides; themeasurement method takes a single reference substance for measuring the total content of compounds with similar structures in the morinda officinalis oligosaccharides in morinda officinalis Chinese herbal medicine or morinda officinalis extract, and simultaneously measuring the ingredients and the relative contents thereof in the measured chemical part, thereby simplifying the operation steps, improving the accuracy of the content measurement, reducing the number of the reference substances, shortening the measurement time, and having good repeatability and high applicability.