The invention discloses a fine-needle puncutre pressure regulator which comprises a needle holder and a pintle buckle that is fastened with the tail end of the pintle of a puncture needle. The pintlebuckle is arranged in the needle holder and can slide back and forth in the needle holder. The fore end of the needle holder is provided with a mounting hole of the puncture needle. The pintle buckleis connected with a host machine which can drive the pintle buckle to slide back and forth in the needle holder. The host machine is connected with a footswitch used for controlling the start-up and the stop of the host machine. The fine-needle puncture pressure regulator has the beneficial effect of realizing the automation of cell collection so that an operator only needs to hold the puncture needle with one hand and control the retraction of the pintle via the footswitch by mechanical or pneumatic principles. The fine-needle puncture pressure regulator has the advantages of accuracy, high collecting rate, less damage on the puncture site of a patient, easy operation and the comfort of the patient.