The invention discloses a method for separating and purifying a flavone compound of momordica grosvenori leaves by HSCCC and the product thereof, wherein, the method of the invention comprises the following steps: the grosvenori leaves are added with water or other polar solvents so as to carry out reflux extraction; the obtained extracts adopt the mixed solution of ethyl acetate, normal butanol and water as a solvent system and are separated and purified by a preparation-type high-speed counter current chromatograph, and then kaempferol-3-O-Alpha-L- rhamnose-7-O-[Beta-D-glucosyl group(1-2)-O-L-rhamnoside] and kaempferol-3,7-Alpha-L-2- rhamnoside. In the method, by applying the HSCCC method to separate and purify the extracts of the momordica grosvenori leaves, two flavone compounds with higher purity are successfully obtained by separation, thereby extending the source of the raw materials for flavone products; moreover, the method has simple preparation technique, easy operation andhigh purification efficiency, lays the solid first stone for extraction and utilization of the effective components in the momordica grosvenori leaves, and can avoid environment pollution caused by the waste and throw of a large amount of momordica grosvenori leaves.