The invention relates to an electro-wetting display and a manufacturing method thereof. The electro-wetting display comprises a first substrate, a second substrate, a plurality of isolation walls arranged on the first substrate in a grid form, a plurality of pixel electrodes, lightproof masking fluid and various dyeing fluids of different colors. The minimum region structured by the adjacent isolation walls defines a sub-pixel unit. The pixel electrodes are arranged on the second substrate correspondingly to the sub-pixel units. The lightproof masking fluid fills in the sub-pixel units, and each dyeing fluid is the mixed solution of the transparent conductive solution and water-soluble dyes or water-soluble pigments; the mixed solution is incompatible with the masking fluid; all the mixedsolutions respectively fill in the sub-pixel units. The incident rays from any substrate are filtered by the dyeing fluids. The electro-wetting display and the manufacturing method thereof can realize full-color display and the manufacturing cost is lower, and manufacturing method is also simpler.