The invention relates to the application of oncolytic adenovirus recombinant and chemotherapeutic medicine of tumor cell special-expression immune adjusting factor GM-CSF in preparing medicine for treating tumor, and the application of the oncolytic adenovirus recombinant in preparing medicine having antineoplastic activity generated in a human body performing ionizing radiation. The adenovirus recombinant is composed of carrier virus, tumor cell special gene regulating element and immunity regulation gene. The oncolytic adenovirus recombinant not only can stimulate the organism immune systemof a tumor patient and weaken the noxious and side effect of part of radiation treatment and chemo-treatment, but also can improve the oxygen lack state of tumor cells, thereby improving the sensibility of the tumor cells for radiation treatment and chemo-treatment and having antitumor synergistic effect.