The invention discloses a production method of valerian iridoid, which comprises the following steps: (1) induction cultivation and treatment of valerian callus: the explant of the valerian is inoculated to an MS solid medium containing corresponding plant growth regulatory substances for culture, successive transfer culture is carried out once every 15 days to 30 days, and the valerian callus containing more iridoid is selected; (2) cell suspension culture: the valerian callus is transferred to the liquid medium for gradual expansion culture and the valerian cells by expansion culture are inoculated to the liquid medium in a bioreactor for large-scale valerian cell suspension culture so as to obtain valerian cells; and (3) the iridoid is obtained by the extraction and separation of the valerian cells. The production method of the valerian iridoid resolves the problem that the production of valerian iridoid is short of raw materials, has high product purity, easy-control technical process and relatively low cost.