The invention provides a method for creating a cut flower cultivar of Gypsophila paniculata which can resist against Liriomyza sativae, which obtains a tissue culture plantlet of genetically modified Gypsophila paniculata which can resist against Liriomyza sativae by constructing a high efficient genetic transformation system of Gypsophila paniculata, sieving the background of Gypsophila paniculata antibiotics, utilizing Bt genes to carry out genetic transformation to the G33 cultivar of Gypsophila paniculata, sieving the antibiotics of transformed plants, carrying out Pcr identification to the transformed plants and the rooting and propagation of the transformed plants. The method effectively improves the capability of Gypsophila paniculata in resisting against Liriomyza sativae; compared with the control plants inoculated in the same period, the morbidity degree of Liriomyza sativae is obviously lowered, thereby indicating that the genetically modified plants can effectively lower the harm of Liriomyza sativae done to Gypsophila paniculata, reduce cost for the culture management of Gypsophila paniculata and have practical meaning to practical planting production.