The invention relates to a method for analyzing heterocyclic amine compounds in mainstream cigarette smoke. The method is characterized in that the analytic method utilizes a cambridge filter to trap the heterocyclic amine compounds in the mainstream cigarette smoke; the heterocyclic amine compounds are extracted by a HCL/ methanol solution, are purified through an MCX ion exchange column, condensed and detected by liquid chromatography-tandem mass spectrometry (HPLC-MS/MS); and the contents of the main heterocyclic amine compounds in the mainstream cigarette smoke are mensurated. Compared with the prior method for analyzing the heterocyclic amine compounds in the mainstream cigarette smoke, the analytic mensuration method does not need a complicated sample treatment step, does not need to derive a sample, has high sensitivity, good repeatability and reclaiming rate, and is suitable for analyzing the samples with large batches.