The invention discloses a drought induced rice flower-specific promoter and application thereof. The promoter has a nucleotide sequence shown in SEQ ID No.1, or a nucleotide sequence which can be hybridized with the sequence under highly rigorous conditions and has same functions. The promoter has flower tissue specificity and shows certain inductivity under drought induction conditions. By constructing a plant expression vector containing the promoter, the promoter is transformed into transgenic rice, the GUS histochemical staining and the RT-PCR detection are performed to a plurality of different tissues of a transgenic material such as ordinary leaves, sword-like leaves, flowers and so on under the drought induction conditions and the result proves the expression of a driving gene of the promoter in a rice flower tissue under the drought induction. The cloning of the promoter has greater practical significance on the research of male sterile strains of rice, and can be used for creating sterile lines of the rice and other plants and maintaining line-double-lines.