The invention relates to a Chinese herbal medicine for the treatment of leukemia, which is prepared by 20 Chinese herbal medicines, namely, 40 portions of buffalo horn, cornucervi pantotrichum, panax ginseng, cortex moutan radicis, fleece-flower root, tortoise plastron, rhizoma ligustici wallichii, radix ophiopogonis, prepared rhizome of rehmannia, angelica, root of herbaceous peony, cape jasminefruit, cherokee rose, rhizoma anemarrhenae, bark of amur corktree, mastic, myrrh, resina draconis, rhizoma cyperi and liquorice. The Chinese herbal medicine has precise matching, moderate severity and priorities, and can effectively cure various leukemia completely and has the advantages of good curative effects, fast onset, short treatment period, high cure rate, no recurrence and the like. Furthermore, all the Chinese herbal medicines used are innoxious and can be used for eliminating diseases and has no toxic side effects and less treatment cost.