The invention discloses a traditional Chinese medicine shadow assisting agent used in B-ultrasound and a preparation method thereof, and the shadow assisting agent comprises various materials with the following weight proportions: 10 to 20 portions of abalone shell, 10 to 20 portions of concha mauritiae, 10 to 20 portions of medicated leaven, 10 to 20 portions of radix codonopsitis, 10 to 20 portions of atractylodes, 10 to 20 portions of tuckahoe, 10 to 20 portions of bletilla striata, 10 to 20 portions of field pennycress, 10 to 20 portions of radix linderae and 10 to 20 portions of radix aucklandiae. The traditional Chinese medicine shadow assisting agent used in B-ultrasound has safe usage, no pain, no wound, no toxicity, no allergy reaction, short checking time, high imaging rate, convenient i usage, easy application and low cost, and provides a new technology and new method for the accurate diagnosis of diseases at early stage, and provides a powerful foundation for the clinical diagnosis, and the traditional Chinese medicine shadow assisting agent is a comparatively ideal novel shadow assisting agent.