The invention discloses a method for culturing intestinal bacteria. The method comprises the steps of putting fresh feces into a test tube of diluent A added with glass beads, blending the materials for more than 3 minutes on a blending device, diluting the materials to the dilution degree between 10<-> and 10<-8> according to a serial dilution method, partitioning a culture medium plate according to different spreading concentration, adding the same amount of diluted mixed solution to subareas respectively, uniformly spreading the mixed solution and putting the plate in a thermostat at 37 DEG C for incubation and culture. The method has the advantages of intuitively reflecting the species and quantity of bacteria in specimens, further researching and analyzing the biochemical characteristics, metabolic characteristics, genetic characteristics and the like of cultured bacteria.