The invention discloses a method for propagating American red-maple. The method comprises the following steps: 1) inoculating a stem segment with knots or shoot tip of annual American red-maple on an initial medium to obtain axillary bud or terminal bud by initiation culture; 2) placing the axillary bud or the terminal bud obtained in the step 1 on an enrichment medium to obtain adventitious bud by multiplication culture; and 3) placing the adventitious bud obtained in step 2 on a rooting medium to obtain American red maple seedling. Compared with other vegetative propagation method, the method of the invention has the advantages that needed test materials are easier to acquire, the controllable error of environmental conditions is small, growing is fast, and the period is short; besides,detached tissue culture can be operated continuously and experimented annually; furthermore, the source of the tissue culture material is singular, and clone ancestral feature is consistent, thereby guaranteeing the inherent excellent characteristics of American red maple.