The invention discloses an ultrahigh-pressure extraction method of honeysuckle flower, wild chrysanthemum and mulberry leaf and the application of an extract thereof, belonging to the technical field of cigarettes. The ultrahigh-pressure extraction method is to arrange the honeysuckle flower, the wild chrysanthemum and the mulberry leaf in an ultrahigh-pressure extraction tank after the mixing and add 5 times the weight of 60 percent-95 percent ethanol; the pressure of the mixture is increased to 100MPa-400MPa, kept for 8 min and then released immediately; then the mixture is filtrated, after being concentrated to 20 percent-30 percent of the original volume, and the filtrate is refrigerated overnight; the concentrate is separated by a centrifuge for 15 min at the speed of 5000 turns per minute; the concentrate is further filtrated, and the obtained filtrate is the mixed extract liquid of the honeysuckle flower, the wild chrysanthemum and the mulberry leaf. 1 percent-5 percent of the extract liquid is added in cigarette paper pulp by or 0.1 per mill-1 percent of the extract liquid is added in tobacco shred. The method has the advantages of simplicity, practicality, convenient operation, time conservation, low cost, and high yield of extracted effective ingredients. In the invention, the cigarettes prepared by the extract liquid have various health care functions.