The invention relates to a simple cell climbing film culture and experimental apparatus. The invention is characterized in that the simple cell climbing film culture and experimental apparatus comprises a base with a rectangular groove for horizontally placing a glass slide, horizontal bayonets positioned above the rectangular groove and arranged in two side walls corresponding to the base, and a tube body, wherein, a through hole is formed on a bottom plate of the base and in the center position corresponding to a glass slide; through grooves communicated with the horizontal bayonets are formed on the surface of the base; a sealing ring is arranged at the bottom opening of the tube body, a pair of side lugs is symmetrically arranged on both sides of the bottom opening of the tube body, the pair of the side lugs can pass through the through grooves and can be rotationally inserted in the horizontal bayonets of the base, and sealing is formed between the bottom opening of the tube body and the glass slide; and the through hole on the bottom plate of the base is positioned in the position where the bottom opening of the tube body is. The invention has the advantages of convenient operation, portability, compactness and high success ratio of experiment.