The invention discloses a construction method of building anti-permeability channels using soil curing agent. The method comprises the following steps: blending soil, a soil curing agent, water and fiber to form a curing soil mixing material; and dividing the curing soil mixing material into dry and solid curing soil and plastic curing soil by the water content of the curing soil mixing material,compacting the dry and solid curing soil with tamp equipment to form an intensive strengthened soil anti-permeability layer, and as for the plastic curing soil, adopting cement mortar spray gun technology to muddy spray curing soil to channel base to form a strengthened soil anti-permeability layer, further laying cement mortar on the surface of the strengthened soil anti-permeability layer to form a protection layer, so that frost heaving and destruction of anti-permeability channels in northern cold area can be effectively avoided. The method can solve the problem in the prior construction method of building anti-permeability channels using soil curing agent, increase construction speed of building anti-permeability channels using the soil curing agent, reduce the construction cost, andprolong the service life of the channels. The invention is of great significance for the development of water-saving irrigation and establishment of conservation-oriented society.