The invention provides an ethoxyl starch 130/0.4 sodium chloride injection, in which, 20-80g of ethoxyl starch 130/0.4 and 5-13g of sodium chloride is include in per litre injection. The invention further provides the preparing method and application of the injection. The ethoxyl starch 130/0.4 in the raw material of the medicine in the invention is an improved product of 'heas' used clinically for treating and preventing hypovolemia, acute normovolemic hemodilution and so on. A better normovolemic therapeutic effect is achieved by optimizing molecular weight and molecular weight distribution, reducing displacement grade and changing displacement manner (C2/C6); it can stays in blood for longer time after intravenous drip, consequently, can increase plasma osmotic pressure, tissue fluid back-flow and blood volume, can improve oxygenation condition in or after major operation evidently, and can increase tissue oxygen partial pressure and improve tissue oxygenation faster and earlier, thereby can satisfy the clinic demand better.