The invention relates to the technical field of food handling. In particular, the invention provides a preparation method for buffalo milk beverage. The buffalo milk is used as the raw material to prepare the milk beverage liquor which has lower alcohol content and rich nutrition value. The preparation method comprises the following steps: (1) buffalo milk is ungreased; (2) accessories and ungreased buffalo milk are well blended, homogenized for 10-20 minutes in the temperature of 55-65 DEG C, added with white granulated sugar, heated for 10-20 min in the temperature of 90-95 DEG C, and cooled till 40 DEG C; the accessories comprise black kerneled rice juice and at least one of oat juice, wort juice or wheat juice; (3) the cooled mixed liquor is added with inebriant microzyme hametz and lactic acid bacteria hametz for fermentation for 20-30 hours in the temperature of 20-35 DEG C; (4) the mixed liquor fermented in the step three is fermented for 1-2 days in the temperature of 0-4 DEG C.