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发明授权
CN101469314B 检测类/抗甲状腺激素化合物的双杂交酵母及检测方法 有权

检测类/抗甲状腺激素化合物的双杂交酵母及检测方法
摘要：
本发明提供一种用于检测样品中类/抗甲状腺激素化合物的双杂交酵母细胞及其制备方法，其中该酵母中含有pGBT9-TR酵母表达质粒和pGAD424-GRIP1酵母表达质粒，其中pGBT9-TR酵母表达质粒包含甲状腺激素受体基因，pGAD424-GRIP1酵母表达质粒包含具有如SEQ ID No.2所示序列的甲状腺激素受体共激活因子基因片段。本发明提供还提供了一种检测样品中类/抗甲状腺激素化合物的生物测试方法。采用本发明酵母及生物测试方法，大大提高检测类/抗甲状腺激素的灵敏度。构建的酵母性状稳定，易于培养、筛选，整个类/抗甲状腺激素效应的筛选过程操作简单，所需样品量少，成本低廉。
摘要（英）：

The invention provides a two-hybrid yeast cell for detecting thyrotropic hormone-like/antithyrotropic hormone compounds in a sample and a preparation method thereof, wherein a yeast contains pGBT9-TR yeast expression plasmids and pGAD424-GRIP1 yeast expression plasmids, wherein the pGBT9-TR yeast expression plasmids contain thyroid hormone receptor genes, and the pGAD424-GRIP1 yeast expression plasmids contain thyroid hormone receptor coactivated factor genetic fragments with the sequence of SEQ ID No.2. The invention also provides a bioassay method for detecting the thyrotropic hormone-like/antithyrotropic hormone compounds in the sample. The yeast and the bioassay method greatly improve the sensitivity of the thyrotropic hormone-like/antithyrotropic hormone compounds. The constructed yeast has stable characteristics and is easy to culture and screen; the screening process of the whole thyrotropic hormone-like/antithyrotropic hormone effect is simple to operate; and the required quantity of the sample is small, and the cost is low.

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